BIOL 255T - Introduction to Stem Cell Techniques

4 units
This laboratory/lecture course exposes students to the theoretical and practical aspects of contemporary stem cell techniques. Students will culture mouse embryonic stem cells on feeders and characterize them using immunofluorescence and flow cytometry, differentiate various lineages and observe genetic modifications. Students will prepare inducible pluripotent stem cells (iPS) from primary cells. This includes both the theoretical and practical aspects of applied stem cell in regenerative approaches as well as models of disease for research purposes. Students are expected to keep a laboratory notebook and document their experiments and results. Additionally, students will present scientific papers on recent topics in stem cell basic research, as published in scientific journals of recent year. The goal of this course will be to provide students with basic experience in methods used in stem cell laboratories, so that they can transition to an internship in a stem cell laboratory (although many of these techniques are applicable in a number of diverse fields), and to provide students with the most current stem cell topics while practicing effective presentation and evaluation skills.

Grading: Graded

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